* corresponding authors

Natsuki Tagawa, Keita Mori, Michinori Koebis, Atsu Aiba, Yuichi Iino, Yousuke Tsuneoka*, Hiromasa Funato*. Activation of lateral preoptic neurons is associated with nest-building in male mice. Scientific Reports  14, 8346, 2024　🔗

Yousuke Tsuneoka*, Hiromasa Funato*. Whole brain mapping of orexin receptor mRNA expression visualized by branched in situ hybridization chain reaction. eNeuro 0474-23.2024, 2024　🔗

Staci J. Kim, Noriko Hotta-Hirashima, Fuyuki Asano, Tomohiro Kitazono, Kanako Iwasaki, Shinya Nakata, Haruna Komiya, Nodoka Asama, Taeko Matsuoka, Tomoyuki Fujiyama, Aya Ikkyu, Miyo Kakizaki, Satomi Kanno, Jinhwan Choi, Deependra Kumar, Takumi Tsukamoto, Asmaa Elhosainy, Seiya Mizuno, Shinichi Miyazaki, Yousuke Tsuneoka, Fumihiro Sugiyama, Satoru Takahashi, Yu Hayashi, Masafumi Muratani, Qinghua Liu, Chika Miyoshi, Masashi Yanagisawa*, Hiromasa Funato*. Kinase signalling in excitatory neurons regulates sleep quantity and depth. Nature online, 2022　🔗

Yousuke Tsuneoka*, Hiromasa Funato*. Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization. Frontiers in Molecular Neuroscience 15:976349, 2022　🔗

Sachine Yoshida*, Hiromasa Funato*. Physical contact in parent-infant relationship and its effect on fostering a feeling of safety. iScience 24, 102721, 2021  🔗 

Yousuke Tsuneoka*, Hiromasa Funato*. Cellular composition of the preoptic area regulating sleep, parental, and sexual behavior. Frontiers in Neuroscience 15, 649159, 2021　🔗

Yousuke Tsuneoka*, Hiromasa Funato*. Modified in situ hybridization chain reaction using short hairpin DNAs. Frontiers in Molecular Neuroscience 13, 75, 2020　🔗

Kimiya Narikiyo, Rumiko Mizuguchi, Ayako Ajima, Momoko Shiozaki, Hiroki Hamanaka, Joshua P. Johansen, Kensaku Mori, Yoshihiro Yoshihara. The claustrum coordinates cortical slow-wave activity. Nature Neuroscience 23, 741-753, 2020  🔗

Sachine Yoshida*, Yoshihiro Kawahara, Takuya Sasatani, Ken Kiyono, Yo Kobayashi, Hiromasa Funato*. Infants show physiological responses specific to parental hugs. iScience 23, 10096, 2020 🔗

Hiromasa Funato. Forward genetic approach for behavioral neuroscience using animal models. Proceedings of the Japan Academy, Ser. B, Physical and Biological Sciences 96, 10-31, 2020 🔗

Miyo Kakizaki, Yousuke Tsuneoka, Kenkichi Takase, Staci J. Kim, Jinhwan Choi, Aya Ikkyu, Manabu Abe, Kenji Sakimura, Masashi Yanagisawa*, Hiromasa Funato*. Differential Roles of Each Orexin Receptor Signaling in Obesity. iScience 20, 1-13, 2019 🔗

Chika Miyoshi, Staci J. Kim, Takahiro Ezaki, Aya Ikkyu, Noriko Hotta-Hirashima, Satomi Kanno, Miyo Kakizaki, Mana Yamada, Shigeharu Wakana, Masashi Yanagisawa*, Hiromasa Funato*. Methodology and theoretical basis of forward genetic screening for sleep/wakefulness in mice. Proc Natl Acad Sci USA 116, 16062-16067, 2019 🔗

Yousuke Tsuneoka. Molecular neuroanatomy of the mouse medial preoptic area with reference to parental behavior. Anatomical Science International 94, 39-52, 2019 🔗

Sachine Yoshida*, Ryuko Ohnishi, Yousuke Tsuneoka, Yuka Yamamoto-Mimura, Reiko Muramatsu, Tadafumi Kato, Hiromasa Funato*, Kumi O. Kuroda*. Corticotropin-releasing factor receptor 1 in the anterior cingulate cortex mediates maternal absence-induced attenuation of Transport Response in mouse pups. Frontiers in Cellular Neuroscience 12, 204, 1-14, 2018 🔗

Tomoyuki Fujiyama, Satoshi Miyashita, Yousuke Tsuneoka, Kazumasa Kanemaru, Miyo Kakizaki, Satomi Kanno, Yukiko Ishikawa, Mariko Yamashita, Tomoo Owa, Mai Nagaoka, Yoshiya Kawaguchi, Yuchio Yanagawa, Mark A. Magnuson, Masafumi Muratani, Akira Shibuya, Yo-ichi Nabeshima, Masashi Yanagisawa, Hiromasa Funato*, Mikio Hoshino*. Forebrain Ptf1a is required for sexual differentiation of the brain. Cell Reports 24, 79-94, 2018 🔗

Zhiqiang Wang, Jing Ma, Chika Miyoshi, Yuxin Li, Makito Sato, Yukino Ogawa, Tingting Lou, Chengyuan Ma, Xue Gao, Chiyu Lee, Tomoyuki Fujiyama, Xiaojie Yang, Shuang Zhou, Noriko Hotta-Hirashima, Daniela Klewe-Nebenius, Aya Ikkyu, Miyo Kakizaki, Satomi Kanno, Liqin Cao, Satoru Takahashi, Junmin Peng, Yonghao Yu, Hiromasa Funato*, Masashi Yanagisawa*, Qinghua Liu*. Quantitative phosphoproteomic analysis of the molecular substrates of sleep need. Nature 558, 435-439, 2018 🔗

Satoko Oda, Yousuke Tsuneoka, Sachine Yoshida, Satomi Adachi-Akahane, Masanori Ito, Masaru Kuroda, Hiromasa Funato. Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex. Journal of Comparative Neurology 526, 1329-1350, 2018 🔗

Yousuke Tsuneoka, Sachine Yoshida, Kenkichi Takase, Satoko Oda, Masaru Kuroda, Hiromasa Funato. Neurotransmitters and neuropeptides in gonadal steroid receptor-expressing cells in medial preoptic area subregions of the male mouse. Scientific Reports 7, 9809, 1-16, 2017 🔗

Yousuke Tsuneoka, Shinji Tsukahara, Sachine Yoshida, Kenkichi Takase, Satoko Oda, Masaru Kuroda, Hiromasa Funato. Moxd1 is a marker for sexual dimorphism in the medial preoptic area, bed nucleus of the stria terminalis and medial amygdala. Frontiers in Neuroanatomy 26, 1-13, 2017 🔗

Hiromasa Funato*, Chika Miyoshi, Tomoyuki Fujiyama, Takeshi Kanda, Makito Sato, Zhiqiang Wang, Jing Ma, Shin Nakane, Jun Tomita, Aya Ikkyu, Miyo Kakizaki, Noriko Hotta-Hirashima, Satomi Kanno, Haruna Komiya, Fuyuki Asano, Takato Honda, Staci J. Kim, Kanako Harano, Hiroki Muramoto, Toshiya Yonezawa, Seiya Mizuno, Shinichi Miyazaki, Linzi Connor, Vivek Kumar, Ikuo Miura, Tomohiro Suzuki, Atsushi Watanabe, Manabu Abe, Fumihiro Sugiyama, Satoru Takahashi, Kenji Sakimura, Yu Hayashi, Qinghua Liu, Kazuhiko Kume, Shigeharu Wakana, Joseph S Takahashi, Masashi Yanagisawa* (* corresponding authors). Forward-genetics analysis of sleep in randomly mutagenized mice. Nature 539, 378-383, 2016 🔗

Kenkichi Takase, Yousuke Tsuneoka, Satoko Oda, Masaru Kuroda, Hiromasa Funato. High-fat diet feeding alters olfactory-, social-, and reward-related behaviors of mice independent of obesity. Obesity 24, 886-894, 2016 🔗

Yousuke Tsuneoka, Kenichi Tokita, Chihiro Yoshihara, Taiju Amano, Gianluca Esposito, Arthur J Huang, Lily MY Yu, Yuri Odaka, Kazutaka Shinozuka, Thomas J McHugh, Kumi O Kuroda. Distinct preoptic-BST nuclei dissociate paternal and infanticidal behavior in mice. EMBO Journal 34, 2652-2670, 2015 🔗

Satoko Oda, Hiromasa Funato, Fumi Sato, Satomi Adachi-Akahane, Masanori Ito, Kenkichi Takase, Masaru Kuroda. A subset of thalamocortical projections to the retrosplenial cortex possesses two vesicular glutamate transporter isoforms, VGluT1 and VGluT2, in axon terminals and somata. Journal of Comparative Neurology 522, 2089–2106, 2014 🔗

Gianluca Esposito, Sachine Yoshida*, Ryuko Ohnishi, Yousuke Tsuneoka, Maria del Carmen Rostagno, Susumu Yokota, Shota Okabe, Kazusaku Kamiya, Mikio Hoshino, Masaki Shimizu, Paola Venuti, Takafumi Kikusui, Tadafumi Kato, Kumi O Kuroda (* co-first author). Infant calming responses during maternal carrying in humans and mice. Current Biology 23, 739-745, 2013 🔗

Yousuke Tsuneoka, Teppo Maruyama, Sachine Yoshida, Katsuhiko Nishimori, Tadafumi Kato, Michael Numan, Kumi O Kuroda. Functional, anatomical, and neurochemical differentiation of medial preoptic area subregions in relation to maternal behavior in the mouse. Journal of Comparative Neurology 521, 1633-1663, 2013 🔗

Hiromasa Funato, Makito Sato, Christopher M. Sinton, Laurent Gautron, S. Clay Williams, Amber Skach, Joel K. Elmquist, Arthur I. Skoultchi, Masashi Yanagisawa. Loss of Goosecoid-like and DiGeorge syndrome critical region 14 in interpeduncular nucleus results in altered regulation of rapid eye movement sleep. Proc Natl Acad Sci USA 107, 18155-18160, 2010 🔗

Hiromasa Funato, Allen L. Tsai, Jon T. Willie, Yasushi Kisanuki, S. Clay Williams, Takeshi Sakurai, Masashi Yanagisawa. Enhanced orexin receptor-2 signaling prevents diet-induced obesity and improves leptin sensitivity. Cell Metabolism 9, 64-76, 2009 🔗